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Unfortunately, spatially coherent radiation severely impairs the interpretation of cross-sectional images and limits its use in medical diagnostics by the presence of speckles in OCT reconstructions. To obtain an appropriate signal-to-noise ratio (SNR) of optical reconstructions, most of the OCT techniques use spatially coherent light with adequately high optical density values. There are currently more than a dozen different ways to exploit low-coherence imaging in competitive scenarios, including time versus Fourier domain detection, full-field versus scanning (confocal) configurations, en face versus B-scan acquisition, and swept-source versus spectrometer-based detection. One such modality is optical coherence tomography (OCT), which is a family of imaging technologies based on low-coherence interferometry. Special attention should be paid to optical methods providing information about the amplitude and phase, which can be further processed by advanced computational techniques to create volumetric reconstructions. The remarkable development of optical imaging methods that has taken place in recent decades brings us closer to noninvasive microscopic imaging of cells embedded in the tissues of living organisms. © 2019 Optical Society of America under the terms of the OSA Open Access Publishing Agreement 1. Introducing crosstalk and speckle-free OCT will advance imaging prospects closer to the ideal of a noninvasive optical biopsy. Additionally, the sample is illuminated under variable angles to reduce the contrast of speckles by incoherent summation of the crosstalk-free volumes. To minimize crosstalk, we implemented a very fast deformable membrane that introduces random phase illumination.
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In this work, we demonstrate for what we believe is the first time how to efficiently reduce crosstalk and enable microscopic quality volumetric reconstructions of the scattering tissue-like human skin in vivo, all within less than a half of a second. The drawback of using parallel detection is that scattered light can travel laterally and get mapped improperly at a camera creating optical crosstalk, which severely impairs the interpretation of subcellular images and limits its use in medical diagnostics.
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Recently, a major advancement toward high-resolution and volumetric imaging was achieved by implementing a parallel detection (i.e., full field) into Fourier-domain optical coherence tomography. Note: Author names will be searched in the keywords field, also, but that may find papers where the person is mentioned, rather than papers they authored.Ĭellular resolution imaging of biological structures has always been a challenge due to strong scattering that limits the achievable transverse resolution or imaging penetration depth.Use a comma to separate multiple people: J Smith, RL Jones, Macarthur.Use these formats for best results: Smith or J Smith.
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For best results, use the separate Authors field to search for author names.Use quotation marks " " around specific phrases where you want the entire phrase only.Question mark (?) - Example: "gr?y" retrieves documents containing "grey" or "gray".Asterisk ( * ) - Example: "elect*" retrieves documents containing "electron," "electronic," and "electricity".
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